Tuesday, March 26, 2019

Shining a light on human acrocentric p-arms


The GRC is excited to announce that representations for the p-arms of the human acrocentric chromosomes can now be found in the GRCh38.p13 patch update of the reference genome, thanks to work done in Brian McStay's lab. These sequences are included on the following scaffolds: ML143366.1, ML143367.1, ML143372.1, ML143377.1, and ML143380.1.

The p-arms of the human acrocentric chromosomes HSA13-15, 21 and 22 each bear ribosomal gene arrays (Figure 1) termed nucleolar organiser regions (NORs). These are the most transcriptionally active regions of the genome and direct formation of nucleoli, the largest structures in the nuclei of all human cells. Research on these critical genomic regions is hampered by the fact that acrocentric p-arms are not included in human genome drafts. They are both internally highly repetitive and share a strikingly similar sequence content, making them recalcitrant to standard sequencing approaches. Despite these issues, Brian McStay's lab previously described a collection of sequenced cosmid and BAC clones that allowed them build a reasonable consensus for sequences both immediately proximal and distal to NORs (Floutsakou et al. 2013. Genome Res 23:2003-12). Proximal sequences are almost entirely segmentally duplicated, similar to regions bordering centromeres. In contrast, the distal sequence is predominantly unique to the acrocentric p-arms. Their interphase localisation, open chromatin structure and transcriptionally active state, point to a role in nucleolar biology and prioritise their inclusion in a future genome draft (for discussion see McStay. 2016 Genes Dev. 30:1598-610).

The McStay lab subsequently developed a workflow that has enabled them to determine the NOR distal sequence, the Distal Junction (DJ) from all five acrocentric chromosomes and from an additional two versions of HSA21, ~3 Mb in all. A panel of mono-chromosomal somatic cell hybrids, mouse A9 cells containing individual human chromosomes, allowed them to sequence one chromosome at a time. Sequencing was performed by combining sequence capture with PacBio SMRT sequencing. Pre-capture libraries (typically in the range of 4-6 kb) were prepared from each hybrid line. Capture was performed using oligonucleotide libraries designed using their original consensus. Circular consensus sequencing (CCS) of post-capture libraries generated so called reads of insert (ROIs) each with high sequence accuracy. This allowed the McStay group to assemble sequence contigs from the NOR distal region of each chromosome, regardless of the presence of repetitive sequences such as satellite DNA.

Their analysis of these sequences confirms sequence and presumably functional conservation between the acrocentrics. It also provides evidence for non-homologous exchanges between them. It's anticipated that extension of sequence contigs towards the telomeres will uncover increased structural variation between the acrocentric chromosomes.

Figure 1. FISH experiment showing the relative locations of the rDNA array and distal junctions on the p-arms of the human acrocentric chromosomes.



46 comments:

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  9. I'm 40 years old female I tested genital herpes (HSV1-2) positive in 2002. I was having bad outbreaks. EXTREMELY PAINFUL. I have try different kinds of drugs and treatment by the medical doctors all to know was avail. Six months ago I was desperately online searching for a helpful remedies for genital herpes (HSV1-2) cure, which i come across some helpful remedies on how Dr OYAGU have help so many people in curing genital herpes (HSV1-2) with the help of herbal treatment because I too believe there is someone somewhere in the world who can cure herpes completely. At of the past 2 months, however, I've been following his herpes protocol Via oyaguherbalhome@gmail.com or +2348101755322 and it stopped all outbreaks completely! To my greatest surprise I was cured completely by following the protocol of his herbal medicine . Don't be discouraged by the medical doctors. There is a cure for HSV with the help of herbs and roots by a herbalist Dr call Dr OYAGU he is so kind and truthful with his herbal treatment, kindly contact him for more information Via oyaguherbalhome@gmail.com or WhatsApp DR on +2348101755322 or vist his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome you will be lucky as i am today

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  11. God bless Dr. USELU for his marvelous work in my life, I was diagnosed of HERPES SIMPLEX VIRUS since 2018 and I was taking my medications, I wasn’t satisfied i needed to get the HERPES out of my system, I searched about some possible cure for HERPES i saw a comment about Dr. USELU , how he cured HERPES with his herbal medicine, I contacted him and he guided me. I asked for solutions, he started the remedy for my health, he sent me the medicine within 3 days. I took the medicine as prescribed by him and 2weeks later i was cured from HERPES contact him via email (dr.uselucaregiver@gmail.com) once again thanks to you Dr. USELU cure the flowing virus, contact his email or add him on whatsapp (+2347052898482) cancer cure
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  13. Hello Am From The UK , I want to write a little testimony about the good work of doctor Oyagu who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc Oyagu email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address oyaguherbalhome@gmail.com or text him on what's app, phone number +2348101755322 or vist his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Dr Oyagu also has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE AND MANY MORE. I oblige everyone to contact this powerful herbalist Dr Oyagu and be free from suffering.

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  14. I already gave up on ever getting cured of HSV2 because i have try many treatment none of them work out for me i have be to different hospital they always tell me same thing there is no cure for herpes when i came across a post about Dr oyagu, in the net from a lady called Rebecca i contacted him and he reassured me with his herbal medicine which i took according to the way he instructed, that how i was cured. I doubted at first because i have been to a whole lot of reputable doctors, tried a lot of medicines but none was able to cure me. so i decided to listen to him and he commenced treatment, and under two weeks i was totally free from Herpes. i want to say a very big thank you to DR oyagu for what he has done in my life. feel free to leave him a message on email oyaguherbalhome@gmail.com and also WhatsApp him +2348101755322.. or vist his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome. He can still be able to help you with this herbs medicine: 1...ALS CURE/DIABETES CURE/EPILESY/HPV CURE/LUPUS/HEPATITIS/CANCER/GOUT

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  15. I already gave up on ever getting cured of HSV2 because i have try many treatment none of them work out for me i have be to different hospital they always tell me same thing there is no cure for herpes when i came across a post about Dr oyagu, in the net from a lady called Rebecca i contacted him and he reassured me with his herbal medicine which i took according to the way he instructed, that how i was cured. I doubted at first because i have been to a whole lot of reputable doctors, tried a lot of medicines but none was able to cure me. so i decided to listen to him and he commenced treatment, and under two weeks i was totally free from Herpes. i want to say a very big thank you to DR oyagu for what he has done in my life. feel free to leave him a message on email oyaguherbalhome@gmail.com and also WhatsApp him +2348101755322.. or vist his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome. He can still be able to help you with this herbs medicine: 1...ALS CURE/DIABETES CURE/EPILESY/HPV CURE/LUPUS/HEPATITIS/CANCER/GOUT

    ReplyDelete
  16. DON'T BELIEVE THERE IS A CURE FOR HERPES? Well here is my testimony about a doctor who helped me when I was infected with HERPES on September 22, 2014, I went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution so that my body can be okay. One day I was online and i saw the testimony of Mrs Amy Kendral speaking on how a man called Dr Razor helped her Eradicate the Herpes Simplex Infection that was eating her up every day, I Immediately emailed the Herbal Doctor Razor with the Details provided by This Woman on how to reach Him and I explained everything to him. Dr Razor told me all the things I needed to do and also gave me instructions to take a wish I followed properly. Before I knew what was happening after 18 days of taking the medication I started having changes in my body, so i told the herbalist of the development in my health and he asked me to go for a medical check up, the Doctors There were Amazed that Herpes have a Permanent and complete cured. Doctor Razor's website :  https://herbalistrazorherb.wixsite.com/drrazorherbalhome. You can Reach him on his Email via drrazorherbalhome@gmail.com . WhatsApp/Call him on +2349065420442. I made sure the Virus wasn't laying dormant in my nerve cells because I have heard of a situation like that. Before deciding to write this testimony so that many who are Battling with different kinds of Challenges Can also get cured.

    ReplyDelete
  17. I want to thank Dr Emu a very powerful spell caster who help me to bring my husband back to me, few month ago i have a serious problem with my husband, to the extend that he left the house, and he started dating another woman and he stayed with the woman, i tried all i can to bring him back, but all my effort was useless until the day my friend came to my house and i told her every thing that had happened between me and my husband, then she told me of a powerful spell caster who help her when she was in the same problem I then contact Dr Emu and told him every thing and he told me not to worry my self again that my husband will come back to me after he has cast a spell on him, i thought it was a joke, after he had finish casting the spell, he told me that he had just finish casting the spell, to my greatest surprise within 48 hours, my husband really came back begging me to forgive him, if you need his help you can contact him with via email: Emutemple@gmail.com or add him up on his whatsapp +2347012841542 is willing to help any body that need his help.

    ReplyDelete
  18. I'm 40 years old female I tested genital herpes (HSV1-2) positive in 2016. I was having bad outbreaks. EXTREMELY PAINFUL. I have try different kinds of drugs and treatment by the medical doctors all to know was avail. Six months ago I was desperately online searching for a helpful remedies for genital herpes (HSV1-2) cure, which I come across some helpful remedies on how Dr OYAGU have help so many people in curing genital herpes (HSV1-2) with the help of herbal treatment because I too believe there is someone somewhere in the world who can cure herpes completely. At of the past 2 months, however, I've been following his herpes protocol Via oyaguherbalhome@gmail.com or +2348101755322 and it stopped all outbreaks completely! To my greatest surprise I was cured completely by following the protocol of his herbal medicine . Don't be discouraged by the medical doctors. There is a cure for HSV with the help of herbs and roots by a herbalist Dr call Dr OYAGU he is so kind and truthful with his herbal treatment, kindly contact him for more information Via oyaguherbalhome@gmail.com or WhatsApp DR on +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome you will be lucky as I am today

    ReplyDelete
  19. Thanks to Doctor Razor for the Great miraculous work he did in my life with his herbal remedy. I have been living with this virus and I have shared with my partners my situation because they deserve to have a choice of whether they want to change being with me or not. I unfortunately did not have a choice in this matter leaving me vulnerable and now faced with this virus for the rest of my life or until a cure was discovered.I refused to take the Medical drugs my PCD have told me to take, I surrendered to my fate, because I was careless. Late on at night, Feb 13 2017, I came across Doctor Razor herbal medicine. I browsed about the authenticity of This Doctor, I got to see several Testimonies regarding his works from people he had helped with his medicine. i reached out to him and told him my problems, after we spoke he promised to help me out. I started using his herbal medicine which he sent to me Via the Courier Delivery, and asked me to take the herbal medicine for 18 days. which i did. I am delighted to let you all know that I Got cured of Herpes Virus, after the Medical Doctor had confirmed this with the test results, when I went for a medical check up after completing the Herbal Dosage Of 18 days. Doctor Razor's Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome or Reach out to Doctor razor via email : drrazorherbalhome@gmail.com WhatsApp +2349065420442.

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  22. I could no longer stand to be deceived while I suffer rejection and pain from my loved ones, I suffered and lost too much from the cruel hand of the nasty HERPES virus 2, I refused to give in to the no cure attitude and that lead me into a great quest for a cure and to the end of my predicament, I wasted time effort and resources until GOD decided to look into my case, Then I stumbled on Some Reviews On how Doctor Razor Has been Saving Lives with his herbal Treatment. I contacted the Herbal Doctor Razor, After visiting his website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome   Who Promised to help me get rid of the virus completely. He assured me of a normal health then gave me the herbal mixture that I used for 18 Days with the Instruction of this herbal doctor Razor, after completing the dosage, I went to the medical Hospital who has been lying to me that there is no cure to the virus since being Diagnosed with the Herpes virus 2 on Nov 17, 2016, to be retested and to my greatest surprise there was no trace of the herpes virus in my system as I speak and subsequent test results have proved it. Since then I've been more careful. I love my life now, It's a new life for me so to say. All thanks to you doctor Razor for helping me. Contact him on his email : drrazorherbalhome@gmail.com . Whatsapp/Call him on +2349065420442

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  23. I have been suffering from (HERPES) disease for the last four years and had constant pain, especially in my knees. During the first year, I had faith in God that I would be healed someday.This disease started to circulate all over my body and I have been taking treatment from my doctor, a few weeks ago I came on search on the internet if I could get any information concerning the prevention of this disease, on my search I saw a testimony of someone who has been healed from (Hepatitis B and Cancer) by this Man Dr. Silver and she also gave the email address of this man and advise we should contact him for any sickness that he would be of help, so I wrote to Dr. Silver telling him about my (HERPES Virus) he told me not to worry that I was going to be cured!! hmm i never believed it,, well after all the procedures and remedy given to me by this man few weeks later I started experiencing changes all over me as the Dr. assured me that I have cured, after some time i went to my doctor to confirmed if I have been finally healed behold it was TRUE, So friends my advice is if you have such sickness or any other at all you can email Dr. Silver (drsilverhealingtemple@gmail.com) sir I am indeed grateful for the help I will forever recommend you to my friends!!! with your lovely Email Address (drsilverhealingtemple@gmail.com or whatsapp on +2348123877102

    ReplyDelete
  24. I Want To Appreciate Dr OYAGU for his great deeds, I Was Diagnosed With type 2 Herpes Virus Last year, And I Was Looking For Solution To Be Cured Luckily I Saw Testimonies On How Dr OYAGU Cure Herpes Virus I Decided To Contact Dr OYAGU I Contacted Him He Prepared A Herbal Medicine Portion And Sent It To Me, I Started The Herbal Medicine For My Health. He Gave Me Step By Step Instructions On How To Apply It, When I Applied It As Instructed, I Was Cured Of This Deadly Herpes Within 2 weeks, I Am Now Herpes Negative. My Brother And Sister I No That There Are So Many People That Have The Same Herpes Virus Please contact Dr OYAGU To Help You Too, And Help Me To Thank Dr OYAGU For Cure Me, I’m Cured By Dr. OYAGU Herbal Medicine, His Contact Email:oyaguherbalhome@gmail.com or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhomeOr Cell Whatsapp Number +2348101755322 thank you

    ReplyDelete
  25. Always, anywhere and anytime, I feel very comfortable now all to the work of Dr Idedia, the Herbalist who prepare for me the remedies to my health challenges. i could feel more comfortable when he explained to me how dangerous it's caring Herpes simplex virus (HSV1&2) with me getting affected easily with Other sexually transmitted infections. Genital sores increases my risk of transmitting or contracting other sexually transmitted infections, including AIDS.
    Newborn infection. ...am a lover who ran into it and had a shock on me after the checkup.
    This has been my earnest prayers for the physician therapist to come in with positive solutions to remedies Herpes and most
    Bladder problems. ...
    Meningitis. ...
    Rectal inflammation (proctitis).
    I was so scared when he says that because I fault viral infections. I'm now free and I thank God for leading me to see others getting cured with Herbs. I decided to try and found out that the best way I would have done was to get rid of it and give my testimony. Glory to God I became free from herpes after I received his medicine from the FEDEX and applied as instructed. Till date I had no SYMPTOMS nor virus to detect of me, I called him and thankful for what he did, and ask if he could cure HPV, he said yes and did the same preparation to my friend for her hpv virus and Recovered from it. Nothing is impossible with Herbs IN CURES, you're the next if you will be willing to apply his medicine and instructions on how to manage.
    Sickness has no right to dwell on us, you can reach him at his email address
    For your immediate response to health. kindly contacted Dr Idedia  WhatsApp  +2349074505296 or E-mail:  dr.idediatraditionalhealinghome@gmail.com visit his websites  https://dr-idedia-traditional-healing-home9.webnode.com/ Or https://sites.google.com/view/dridediatraditionalhealinghome/ Or
       https://dr-idedia-traditional-healing-home.business.site/  

    ReplyDelete
  26. I want to use this opportunity to thank Dr Oyagu for the grate work he has done for me and my cousin sister, I was cured from herpes virus through his herbal remedy and my cousin sisters was also cured from Cancer. This is a grate testimony for what Dr Oyagu Has done for me and my entire generation. I’m giving this testimony so that those of you out there can contact him while he keeps saving my relatives. Send him a Dm and get a better health totally. Contact him via WhatsApp +2348101755322 or Email address:oyaguherbalhome@gmail.com you can also reach him on his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome

    ReplyDelete
  27. I Never Used To Believe Herbal Medicine Not Until I Was Affected With #Cholera After Taking So Many Medication And Vaccine But All To Know Avail.If Not For My Co-worker Who Saw A Testimony On How A Herbalist Dr Cure Her Of #Herpes Disease With His herbal Medication.Not Withstanding I Decided to Give Him a Try After I Contacted Him Through His Email And Explained My Predicament to Him,After the Explanation He Ask Me to Give Him Some Days To Prepare The Medicine.
    A Week Interval I Received My Medicine And Ask Me To Follow The Instructions Below the Medicine just (1)Week and (3)Days After Using Behold I Totally Got Cure of CHOLERA.
    You Can Also Reach Him For Any Health Challenge I Recommend Him For You Email 📧
    ogodoherbalhomesolution@gmail.com
    WhatsApp him +2349044680467
    He also specialize in treating all kinds of virus diseases such as
    * SMALL POX
    *YELLOW FEVER
    *SCALET FEVER
    *TYPHOID
    *MEASLES
    *DIPHTHERIA
    *WHOOPING COUGH
    *HIV/AIDS
    *POLIO DISEASE E.T.C
    The Father of Our Time May God Continue To Strengthened you.
    Check his website on his Facebook page https://www.facebook.com/oogoherbalhomesolution/

    ReplyDelete
  28. Hello Am From The UK , I want to write a little testimony about the good work of doctor Oyagu who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc Oyagu email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address oyaguherbalhome@gmail.com or text him on what's app, phone number +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Dr Oyagu also has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE AND MANY MORE. I oblige everyone to contact this powerful herbalist Dr Oyagu and be free from suffering.

    ReplyDelete
  29. HOW DR AZIBA HELPED ME ENLARGE MY PENIS SIZE FROM 3.5 INCHES TO 11INCHES LONG AND 8.0 GROWTH WhatsAPP DR [ +2348100368288 ]
    I got married 2 years ago and it just seemed that there was no excitement in my sex life. My dysfunction to perform to the best of my abilities in bed made it harder for my wife and me to have a good time during sex. And i was having the feelings that she may decide to get a divorce one day. I knew something had to be done in order to improve my sex life and to save my marriage because my marriage was already falling apart, so when i was on my Facebook page i came across a story of how Dr Aziba helped him enlarged his penis to 8ins better.so i Immediately copied the Email address of the Dr and explained to him my problem, he gave me some simply instruction which i must follow and i did easily and my friends Today, i am the happiest man on Earth, All Thanks to Dr Aziba for saving my marriage and making me a real man today. I asked him about some popular diseases like HERPES,HIV AIDS,HEPATITES,DIABETIS, CANCERS, WART, HPV etc.. which are affecting most people he said there is no disease without a cure, he has it all...You can as well reach the Dr below for help on your problem, for he has the solution to all...
    Email:[ PRIESTAZIBASOLUTIONCENTER@GMAIL COM ]
    WhatsAPP DR [ +2348100368288 ]

    ReplyDelete
  30. HOW DR AZIBA HELPED ME ENLARGE MY PENIS SIZE FROM 3.5 INCHES TO 11INCHES LONG AND 8.0 GROWTH WhatsAPP DR [ +2348100368288 ]
    I got married 2 years ago and it just seemed that there was no excitement in my sex life. My dysfunction to perform to the best of my abilities in bed made it harder for my wife and me to have a good time during sex. And i was having the feelings that she may decide to get a divorce one day. I knew something had to be done in order to improve my sex life and to save my marriage because my marriage was already falling apart, so when i was on my Facebook page i came across a story of how Dr Aziba helped him enlarged his penis to 8ins better.so i Immediately copied the Email address of the Dr and explained to him my problem, he gave me some simply instruction which i must follow and i did easily and my friends Today, i am the happiest man on Earth, All Thanks to Dr Aziba for saving my marriage and making me a real man today. I asked him about some popular diseases like HERPES,HIV AIDS,HEPATITES,DIABETIS, CANCERS, WART, HPV etc.. which are affecting most people he said there is no disease without a cure, he has it all...You can as well reach the Dr below for help on your problem, for he has the solution to all...
    Email:[ PRIESTAZIBASOLUTIONCENTER@GMAIL COM ]
    WhatsAPP DR [ +2348100368288 ]

    ReplyDelete
  31. I want to tell everyone how i was cured of HERPES Simplex Virus by a Doctor called Dr Oje Abacha, i visited different hospital but there was no solution until one day i was browsing through the Internet searching for solution and i saw comment of people talking about how Dr Oje Abacha cured them. When I contacted him he gave me hope and sent a Herbal medicine to me that I took and it seriously worked for me,i'm free now without any problem, my HERPES result came out negative. I pray for you Dr Oje Abacha God will give you everlasting life, you shall not die before your time for being a sincere and great man. I'm so happy. You can also  email him at:  oje.herbalsolutioncentre@gmail.com  Or contact him on whatsapp  via +2348159734766 . or Visit his website  https://ojeherbalsolutioncentre.wordpress.com.

    ReplyDelete
  32. TESTIMONY ON HOW I GOT CURED TOTALLY FROM HERPES VIRUS BY DR ISHIAKU 
    I want to inform the public how i was cured of herpes  Virus by a Doctor called Dr ishiaku. I visited different hospitals but they gave me a list of drugs like Famvir, Zovirax, and Valtrex which are very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedies on Herpes and I saw comments of people talking about how Doctor ishiaku cured them. I Was scared because i never believed in the Internet but i was convinced to give him a try because i was having no hope of been cured of herpes so i decided to contact him on his email, i searched his email on net and i saw a lot of people testifying about his goodness. When I contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HERPES result came out negative. You can contact him on his email: ishiakuherbalcure@gmail.com or whats-app him via + 2348180828544 and get all your problems solved.1)Sickness of any kind
    2)Help to CURE Hepatitis B, herpes, HIV/AIDS CANCER and BIPOLAR
    3)Divorce, Breakup problem and To Re-unit
    4)Pregnancy problem
    5)Financial problem and Job promotion
    6)bad mouth odour
    7)eye or ear problem
    8) enlargement of manhood 
    you can also visit his website for information www.ishiakuherbalcure.wordpress.com  

    ReplyDelete
  33. I'm really happy that i have been cured from HSV1&2 DISEASE with the herbal medicine of DR.MOMOH have been suffering from this disease for a long time now without solution until i came across the email of this doctor who have cure so many people with his herbal medicine, i also choose to give him a chance to help me, he told me what to do and i kindly did it, and he gave me his herbal medicine and direct me on how to use it, i also follow his instructions for use and he ask me to go for a check up after 3 months which i did, to my greatest surprise my result came out as negative, i am really happy that there is someone like this doctor who is ready to help anytime any day. To all the readers and viewers that is doubting this testimony stop doubting it and contact this doctor if you have any disease and see if he will not actually help you. I know that there are some people out there who are really suffering and hurting their family just because of these diseases. you can get to him through email: DRMOMOHHEALERTEMPLE@EMAIL.COM or WhatsApp him +2348166979481.

    ReplyDelete
  34. Website: (https://emutemple.wordpress.com/  I was born and raised in a small town on Long Island in New York. I live there today with my wife and three wonderful children. I want to testify of how I was cured from prostate cancer by Dr Emu. I was on hormone therapy for advanced prostate cancer. At first I was having lots of signs and symptoms like excessive urine at midnight and also I was experiencing dribbling of urine all the time I was going through hell I search for a cure☑ on the internet I came to know about Dr Emu I saw lots of testimonies of how he cures diseases like *Prostate Cancer *Herpes  Virus *Genital herpes *Hiv/Aids*Breast Cancer and more am happy am cured thanks to Dr Emu I recommend Dr Emu roots and herbs as best natural herbal medicine with no side effects. For consultation and more contact him via Email: (emutemple@gmail.com) (emutemple@yahoo.com) WhatsApp or call +2347012841542 

    ReplyDelete
  35. Hello, I want to write a little review about the good work of doctor Ojamo, who cured me from HSV 1&2 for just 2 week with his herbal medicine, I never believe I can be cured again and have a good life like others, I always regretted the day I got diagnose with this virus, I become lost of hope when my doctor told me there is no cure for it, Well I am so grateful for my helper who get me cured with his herbal medicine, I went online in search of anything that can help me because I can’t deal with it forever so I found dr Ojamo email dr.ojamoherbalhome@gmail.com on a blog of someone who was cured by him,of the same herpes, so I quickly contacted him for help and explained all my pains to him, he told me not to worry about it there is a cure for real, I never believe until he sent me the herbs when I ordered for it and I got it within 5 working days that is how I took the medicine for 2 weeks and the following week I went for a test just to confirm I was 100% cured from this sickness what a miracle in my life, I am so happy right now, and forever greatful, you can also get in contact with him if you have such sickness through his whatsapp +2349077406037 or email address:: dr.ojamoherbalhome@gmail.com

    ReplyDelete
  36. My husband and I have been married for over 10 years. We met when I was 18 and he was 21. We’ve been through a lot emotionally together. There were several HUGE fights and painful situations in our marriage, but we always seemed to come out stronger on the other side. Out of the blue my husband just sprung the divorce talk on me, I was totally depressed until I found Dr.Todd website online and I ordered for a Love spell. You won’t believe my husband called me at the exact time this spell caster finished his spell work in 48hrs. I was totally amazed! He is wonderful and his spells work so fast. His Email: manifestspellcast@ gmail. com

    ReplyDelete
  37. I am so Happy to be writing this review here, i am here to explore blogs forum about the wonderful and most safe cure for HERPES SIMPLEX VIRUS. I was tested positive to the deadly Virus, HERPES and i lost hope completely because i was rejected even by my closet friends. i searched online to know and inquire about cure for HERPES and i saw Dr Ojamo testimony online on how he has cured so many persons from Herpes Disease so i decided to contact this great herbalist because i know that nature has the power to heal everything. i contacted him to know how he can help me and he told me never to worry that he will help me with the natural herbs from God! after 2 days of contacting him, he told me that the cure has been ready and he sent it to me via FEDEX and it got to me after 4 days! i used the medicine as he instructed me (MORNING and EVENING) and i was cured! its really like a dream but i'm so happy! that i was cured. that's the reason i decided to also add more comment of Him so that more people can be saved just like me and if you need his help contact his Email: dr.ojamoherbalhome@gmail.com you can also contact him on mobile number and via whatsapp +2349077406037

    He have the herb to cure difference cure for any sickness

    (1) HERPES,

    (2) DIABETES,

    (3) HIV&AIDS,

    (4) URINARY TRACT INFECTION,

    (5) HEPATITIS B,

    (6) IMPOTENCE,

    (7) BARENESS/INFERTILITY

    (8) DIARRHEA

    (9) ASTHMA...

    once again emial him via dr.ojamoherbalhome@gmail.com

    ReplyDelete
  38. I was diagnose with human papillomavirus (HPV) 4 years ago and i met a doctor he told me there is no cure and i have be taking some medication the warts multiplied when trying to removed them and it got me embarrass and pains, luckily few weeks ago i was recommend to Dr Ogedegbe for natural treatment after one week i got 100% cure and all the wart and pains got disappeared, i shared this post because its part of my life experience, incase anyone need speedy help about this cure email Dr Ogedegbe via dr.ogedegbe6@gmail.com or Whatsapp him now on +2348109374702 .... or visit his youtube channel https://www.youtube.com/channel/UC-Hx8eErDaQ3gZce2U87GSQ

    ReplyDelete
  39. My Ex-boyfriend left me and my daughter since 6 weeks ago after, I tried almost  everything  to restore my relationship and I was disappointed. None was working out, A week later I saw Dr. Paul website and after I contact Dr.  Paul   for help, everything automatically change, my sadness became joy, smile was all over my face, everything happened very fast  and the result was effective. Dr  Paul   is the only  spell caster who i had success with, my boyfriend called me and said she wanted us to come back and also want to marry me. he came back to me begging me to accept him back and this happened Due to the help of Dr.  Paul  . I Am so happy that I have my boyfriend back. Contact Dr.  Paul   now for an urgent and fast love spell to win your relationship back. Email him: astoriashrine@gmail.com

    ReplyDelete
  40. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442This testimonial serves as an expression of my gratitude.
    He also have a herbal cure for COLD SORE,
    SHINGLES,
    CANCER(Cannabis Oil),
    HPV,
    ASTHMA,
    IMPOTENCE,
    BARENESS/INFERTILITY ..
    Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

    ReplyDelete