Wednesday, March 20, 2019

GRCh38.p13 has been released


The GRC is pleased to announce that GRCh38.p13 is now available! This release adds 45 new scaffolds: 43 FIX patches and 2 NOVEL patches. The FIX patch scaffolds provide assembly corrections while the NOVEL patch scaffolds deliver new alternate sequence representations. A valuable contribution to this patch release comes in the addition of the Nucleolus Organiser Region (NOR) sequences for the short arms of the acrocentric chromosomes (13, 14, 15, 21, and 22) as provided by Brian McStay's group (PMID: 23990606). The NOR additions will be discussed in detail in a separate blog.

With access to an ever-increasing pool of high quality, long-read human assembly data, the GRC has been able to utilise this in GRCh38.p13 to address genome issues that have until now persisted due either to lack of data, or complexity. Much of the data added in this patch is derived from the CHM1 human haploid hydatidiform mole assembly (GCA_001297185.2). Originally produced as part of an assembly comparison analysis (see PMID: 
28396521), the assembly was recently Pilon corrected and re-submitted to GenBank by the McDonnell Genome Institute at the Washington University, a GRC center, with the specific aim of improving the base pair accuracy for use of its sequences in improving the Human Genome Reference.

In GRCh38.p13, a total of 28 assembly gaps have been closed. These updates, together with sequences added to correct 5 clone errors, add more than 0.5 Mb of unique data to the assembly. The majority of unique sequences added in this release come from contigs that are components of WGS assemblies derived from PacBio sequence reads, such as the CHM1 assembly mentioned above. However, genomic clone libraries still play an important role in assembly curation. In this release BAC clones from human cell line (CHM1htert) have provided complete, single haplotype representations of clinically important regions such as Prader-Willi on chromosome 15, and CT47 on chromosome X.

The CT47 cancer/testis antigen located on human Xq24 is organized as an array of 4.8 kb tandemly repeated units. Due to the repetitive nature of the sequence involved, coupled with the limitations of the technologies available at the time, the representation of the CT47 gene cluster in GRCh37 and GRCh38 was problematic. The region is gapped, and the flanking clones are from different haplotypes. As a consequence, the representation of the cluster in these assemblies was incomplete and biologically unsound, representing an indeterminate number of gene copies (Figure 1, top).
Studies have indicated that this polymorphic array is highly variable between haplotypes and ranges from 4 to 17 copies in length. Long-read sequencing of genomic clones has now captured the complete CT47 cluster as a single haplotype. The fix patch (ML143381.1) included in the GRCh38.p13 release now provides a contiguous and validated representation of the CT47 genomic region. This patch closes the assembly gap with sequences from BAC clone AC275592.1 (CH17-182I12) which contains a complete, 7 copy representation of the CT47 array (Figure 1, bottom). Note that this update reduces the number of CT47 genes represented as compared to GRCh37 and GRCh38.
Figure 1 Top: CT47 region in GRCh38. Incomplete representation of CT47 gene cluster in GRCh38 due to an assembly gap. Bottom: CT47 fix patch in GRCh38.p13. The gap is closed and a complete representation of CT47 cluster is provided. 
Optical mapping technology has been used to confirm the copy number for the CT47 array is accurate for the haploid CHM1tert sample (Figure 2), from which the clone library was derived.
Figure 2: AC275592.1 alignment to CHM1 Bionano optical map.

As more data becomes available using the latest technologies the GRC is able to utilise this in order to continually to update and improve the reference genome. If you have questions about this process, let us know.

You can download the GRCh38.p13 assembly, including the alignments of the patches to GRCh38, from the GenBank FTP.


51 comments:

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  8. God bless Dr. USELU for his marvelous work in my life, I was diagnosed of HERPES SIMPLEX VIRUS since 2018 and I was taking my medications, I wasn’t satisfied i needed to get the HERPES out of my system, I searched about some possible cure for HERPES i saw a comment about Dr. USELU , how he cured HERPES with his herbal medicine, I contacted him and he guided me. I asked for solutions, he started the remedy for my health, he sent me the medicine within 3 days. I took the medicine as prescribed by him and 2weeks later i was cured from HERPES contact him via email (dr.uselucaregiver@gmail.com) once again thanks to you Dr. USELU cure the flowing virus, contact his email or add him on whatsapp (+2347052898482) cancer cure
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  10. God bless Dr. USELU for his marvelous work in my life, I was diagnosed of HERPES SIMPLEX VIRUS since 2018 and I was taking my medications, I wasn’t satisfied i needed to get the HERPES out of my system, I searched about some possible cure for HERPES i saw a comment about Dr. USELU , how he cured HERPES with his herbal medicine, I contacted him and he guided me. I asked for solutions, he started the remedy for my health, he sent me the medicine within 3 days. I took the medicine as prescribed by him and 2weeks later i was cured from HERPES contact him via email (dr.uselucaregiver@gmail.com) once again thanks to you Dr. USELU cure the flowing virus, contact his email or add him on whatsapp (+2347052898482) cancer cure
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  11. DR OYAGU herbal medicine is a good remedy for herpes simplex virus cure. My name is MIGNON WRIGHT , am from USA,I am here to give my testimony about DR OYAGU who helped me in my life, i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by DR OYAGU, i visited different hospital but they gave me list of drugs like Familiar, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how DR OYAGU cured them, when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without any problem, my HERPES result came out negative, I pray to God to never let me and my family forget you in our life,am now cured permanently, you can also get your self cured my friends if you really need this doctor help, you can reach him now his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Email: oyaguherbalhome@gmail.com or via whatsapp +2348101755322

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  13. DR OYAGU herbal medicine is a good remedy for herpes simplex virus cure. My name is MIGNON WRIGHT , am from USA,I am here to give my testimony about DR OYAGU who helped me in my life, i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by DR OYAGU, i visited different hospital but they gave me list of drugs like Familiar, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how DR OYAGU cured them, when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without any problem, my HERPES result came out negative, I pray to God to never let me and my family forget you in our life,am now cured permanently, you can also get your self cured my friends if you really need this doctor help, you can reach him on his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Email: oyaguherbalhome@gmail.com or via whatsapp +2348101755322

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  14. I already gave up on ever getting cured of HSV2 because i have try many treatment none of them work out for me i have be to different hospital they always tell me same thing there is no cure for herpes when i came across a post about Dr oyagu, in the net from a lady called Rebecca i contacted him and he reassured me with his herbal medicine which i took according to the way he instructed, that how i was cured. I doubted at first because i have been to a whole lot of reputable doctors, tried a lot of medicines but none was able to cure me. so i decided to listen to him and he commenced treatment, and under two weeks i was totally free from Herpes. i want to say a very big thank you to DR oyagu for what he has done in my life. feel free to leave him a message on email oyaguherbalhome@gmail.com and also WhatsApp him +2348101755322.. or vist his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome. He can still be able to help you with this herbs medicine: 1...ALS CURE/DIABETES CURE/EPILESY/HPV CURE/LUPUS/HEPATITIS/CANCER/GOUT

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  15. Herpes Virus is a very terrible virus, if not for my Husband and the help of the great Dr. OSE my life would have been a mess! I was diagnosed with Herpes virus in 2014, my husband encouraged me, and told me not to lose hope, I managed to give birth to a baby Boy who was free from this virus, and my Husband was always there for me! One day he came to me and told me that he had found a man who could cure me. Based on scientists, they said there is no cure for Herpes virus; he told me that he has seen many testimonies about him on the internet. We decided to contact him, we filled his Herbal home form, and he asked us to buy some local root and herbs items which we did! and he prepared for me a herbal medicine and 14 days later he asked me to go for a medical test, faithfully hopefully i went for the medical test, and Behold, I was cured from this virus and it was not found in my Body system again... My Dear Husband and my Father Dr. OSE, God will surely bless the both of you till the end of time, in Jesus name.. If you have any problem kindly contact via his Email: droseherbalhome@gmail.com..

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  16. It’s obvious some patients with herpes are being enslaved to the antiviral and other supplementary Orthodox medicine just to help suppress the virus and not a cure. I have been with the virus since 2015 until I was introduced by a blogger who also narrated her story online on how she was cured of Genital Herpes after using Dr Sikies Herbal Medicine. This is a year and 2 weeks since I was delivered from genital Herpes. All thanks to God for using this Great herbalist to heal me. I have promised to keep telling good things about Dr Sikies. Please feel free to share Your problems with him and don’t forget to tell him I did refer you to Him. Thanks. Write him on mail Drsikies@gmail.com He deals with Alzheimer virus, Cancer, HIV, Herpes, Genital, warts, ALS, BV, UTI, Virginal infection, Genital, Wart, HPV, Hepatitis A/B, Good luck, HSV, Pregnancy, Ex back.  

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  17. Good news this is to everyone out there with different health challenges, as I know there are still a lot of people suffering from different health issues and are therefore looking for solutions. I bring you Good news. There is a man called Dr ehiaguna a herbal practitioner who helped cure me from HSV (2), i had suffered from this diseases for the past 5 years and i have spent so much money trying to survive from it. I got my healing by taking the herbal medicine Dr ehiaguna sent to me to drink for about 14 days . 3 days after completion of the dosage, I went for a medical checkup and I was tested free from HSV. all thanks to God for leading me to Dr ehiaguna who was able to cure me completely from this deadly diseases, I’m sharing this so that other people can know of this great healer called Dr ehiaguna because I got to know him through elizabeth who he cured from HIV. I was made to understand that he can cure several other deadly diseases and infections. Don’t die in ignorance or silent and don’t let that illness take your life. Contact Dr ehiaguna through his email  drehiaguna@gmail.com You can also whatsapp/call him on:+2348073908953 . Visit his website  https://effectiveherbalcurehome.blogspot.com/   He cure all forms of disease {1}HIV/AIDS {2}DIABETES {3}EPILEPSY {4} BLOOD CANCER {5} HPV {6} BRAIN TUMOR {7} HEPATITIS {8}COPD{9} SICKLE AND ANAEMIA.etc Be kind enough to share as you received.  

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  18. DON'T BELIEVE THERE IS A CURE FOR HERPES? Well here is my testimony about a doctor who helped me when I was infected with HERPES on September 22, 2014, I went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution so that my body can be okay. One day I was online and i saw the testimony of Mrs Amy Kendral speaking on how a man called Dr Razor helped her Eradicate the Herpes Simplex Infection that was eating her up every day, I Immediately emailed the Herbal Doctor Razor with the Details provided by This Woman on how to reach Him and I explained everything to him. Dr Razor told me all the things I needed to do and also gave me instructions to take a wish I followed properly. Before I knew what was happening after 18 days of taking the medication I started having changes in my body, so i told the herbalist of the development in my health and he asked me to go for a medical check up, the Doctors There were Amazed that Herpes have a Permanent and complete cured. Doctor Razor's website :  https://herbalistrazorherb.wixsite.com/drrazorherbalhome. You can Reach him on his Email via drrazorherbalhome@gmail.com . WhatsApp/Call him on +2349065420442. I made sure the Virus wasn't laying dormant in my nerve cells because I have heard of a situation like that. Before deciding to write this testimony so that many who are Battling with different kinds of Challenges Can also get cured.

    ReplyDelete
  19. I want to thank Dr Emu a very powerful spell caster who help me to bring my husband back to me, few month ago i have a serious problem with my husband, to the extend that he left the house, and he started dating another woman and he stayed with the woman, i tried all i can to bring him back, but all my effort was useless until the day my friend came to my house and i told her every thing that had happened between me and my husband, then she told me of a powerful spell caster who help her when she was in the same problem I then contact Dr Emu and told him every thing and he told me not to worry my self again that my husband will come back to me after he has cast a spell on him, i thought it was a joke, after he had finish casting the spell, he told me that he had just finish casting the spell, to my greatest surprise within 48 hours, my husband really came back begging me to forgive him, if you need his help you can contact him with via email: Emutemple@gmail.com or add him up on his whatsapp +2347012841542 is willing to help any body that need his help.

    ReplyDelete
  20. I'm 40 years old female I tested genital herpes (HSV1-2) positive in 2016. I was having bad outbreaks. EXTREMELY PAINFUL. I have try different kinds of drugs and treatment by the medical doctors all to know was avail. Six months ago I was desperately online searching for a helpful remedies for genital herpes (HSV1-2) cure, which I come across some helpful remedies on how Dr OYAGU have help so many people in curing genital herpes (HSV1-2) with the help of herbal treatment because I too believe there is someone somewhere in the world who can cure herpes completely. At of the past 2 months, however, I've been following his herpes protocol Via oyaguherbalhome@gmail.com or +2348101755322 and it stopped all outbreaks completely! To my greatest surprise I was cured completely by following the protocol of his herbal medicine . Don't be discouraged by the medical doctors. There is a cure for HSV with the help of herbs and roots by a herbalist Dr call Dr OYAGU he is so kind and truthful with his herbal treatment, kindly contact him for more information Via oyaguherbalhome@gmail.com or WhatsApp DR on +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome you will be lucky as I am today

    ReplyDelete
  21. Thanks to Doctor Razor for the Great miraculous work he did in my life with his herbal remedy. I have been living with this virus and I have shared with my partners my situation because they deserve to have a choice of whether they want to change being with me or not. I unfortunately did not have a choice in this matter leaving me vulnerable and now faced with this virus for the rest of my life or until a cure was discovered.I refused to take the Medical drugs my PCD have told me to take, I surrendered to my fate, because I was careless. Late on at night, Feb 13 2017, I came across Doctor Razor herbal medicine. I browsed about the authenticity of This Doctor, I got to see several Testimonies regarding his works from people he had helped with his medicine. i reached out to him and told him my problems, after we spoke he promised to help me out. I started using his herbal medicine which he sent to me Via the Courier Delivery, and asked me to take the herbal medicine for 18 days. which i did. I am delighted to let you all know that I Got cured of Herpes Virus, after the Medical Doctor had confirmed this with the test results, when I went for a medical check up after completing the Herbal Dosage Of 18 days. Doctor Razor's Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome or Reach out to Doctor razor via email : drrazorherbalhome@gmail.com WhatsApp +2349065420442.

    ReplyDelete
  22. I was diagnosed of HERPES Virus in 2018 and I have tried all I can to get cured but all to no avail, until i saw a post in a health forum about a herbalist man who prepare herbal medication to cure all kind of diseases including HERPES virus, at first i doubted if it was real but decided to give it a try , when i contact this herbalist and he prepared a HERPES herbal cure and sent it to me via FEDEX delivery company service, when i received this herbal cure, he gave me step by directions on how to apply it, when i applied it as instructed, i was totally cured of this horrible disease within 14days of usage, i went to the hospital for a test and it was negative. I can tell to the public now that am now free from the horrible disease called herpes, all thanks to Dr.ASAKA that is why I want to leave his email address and What's app contact just if you want to contact him for help. you reach this great herbal doctor via his email on (drasakanaturalremedies@gmail.com)website on: https://drasakanaturalremediesherbalcure.wordpress.com/ One thing I like about Dr ASAKA is honesty. His very polite with his customers, everything he told me was what he did. His herbal medicine are very affordable, effective, confidential.

    ReplyDelete
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    ReplyDelete
  25. Hello my name is Linda i want to share my amazing experience with the greatest spell caster Dr irekenagba. my husband was cheating on me and when i found out we had a fight which lead to him filling for a divorce i cried and fell sick when i was searching about love quotes online i saw people talking about him and his great work whose case was similar to mine they left his contact info i contacted him and he told me not to worry that after 24hrs he will cancel the divorce and be back to me after i did everything he asked me to do to my greatest surprise the next day evening it was my husband he knelt down begging me to accept him back,thank you once again Dr irekenagba you are indeed a blessing to me he can also help you contact him on........
    WhatsApp: +2347059630655
    email: doctorirekenagba@gmail.com

    ReplyDelete
  26. Hello my name is Linda i want to share my amazing experience with the greatest spell caster Dr irekenagba. my husband was cheating on me and when i found out we had a fight which lead to him filling for a divorce i cried and fell sick when i was searching about love quotes online i saw people talking about him and his great work whose case was similar to mine they left his contact info i contacted him and he told me not to worry that after 24hrs he will cancel the divorce and be back to me after i did everything he asked me to do to my greatest surprise the next day evening it was my husband he knelt down begging me to accept him back,thank you once again Dr irekenagba you are indeed a blessing to me he can also help you contact him on........
    WhatsApp: +2347059630655
    email: doctorirekenagba@gmail.com

    ReplyDelete
  27. I could no longer stand to be deceived while I suffer rejection and pain from my loved ones, I suffered and lost too much from the cruel hand of the nasty HERPES virus 2, I refused to give in to the no cure attitude and that lead me into a great quest for a cure and to the end of my predicament, I wasted time effort and resources until GOD decided to look into my case, Then I stumbled on Some Reviews On how Doctor Razor Has been Saving Lives with his herbal Treatment. I contacted the Herbal Doctor Razor, After visiting his website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome   Who Promised to help me get rid of the virus completely. He assured me of a normal health then gave me the herbal mixture that I used for 18 Days with the Instruction of this herbal doctor Razor, after completing the dosage, I went to the medical Hospital who has been lying to me that there is no cure to the virus since being Diagnosed with the Herpes virus 2 on Nov 17, 2016, to be retested and to my greatest surprise there was no trace of the herpes virus in my system as I speak and subsequent test results have proved it. Since then I've been more careful. I love my life now, It's a new life for me so to say. All thanks to you doctor Razor for helping me. Contact him on his email : drrazorherbalhome@gmail.com . Whatsapp/Call him on +2349065420442

    ReplyDelete
  28. I'm here to testify about the great work Dr Oluta did for me. I have been suffering from (HERPES) disease for the past 5 years and had constant pain, especially in my knees. During the first year,I had faith in God that i would be healed someday.This disease started circulating all over my body and i have been taking treatment from my doctor, few weeks ago i came across a testimony of one lady on the internet testifying about a Man called Dr Oluta on how he cured her from Herpes Simplex Virus. And she also gave the email address of this man and advise anybody to contact him for help for any kind of sickness that he would be of help, so I emailed him on dr.olutaspellcaster@gmail.com telling him about my (HERPES Virus) he told me not to worry that i was going to be cured!! Well i never believed it,, well after all the procedures and remedy given to me by this man few weeks later i started experiencing changes all over me as Dr Oluta assured me that i will be cured,after some time i went to my doctor to confirmed if i have be finally healed behold it was TRUE, So friends my advise is if you have such sickness or any other at all you can contact Dr Oluta via email dr.olutaspellcaster@gmail.com. You can also call or whatsApp his telephone number on +2349065326267 . Thanks once again Dr Outa.  
      https://m.facebook.com/pages/category/Product-Service/Drolutaherbalhome-100710878112591/  

    ReplyDelete
  29. My name is Clifford John from USA taxes, I want to share a testimony of how Dr_Ogodo1 herbal mixture cream saves me from shame and disgrace, my penis was a big problem to me as the size was really so embarrassing,and i was also having weak erection problem. I can't make love to my wife and my penis was just too small a full grown man like me having 4 inches penis and to worsen it i don't last in sex i can't even last two minutes it was really a thing of shame to me. My wife was really tired of me because my sex life was very poor,she never enjoyed sex,i was always thinking and searching for solutions everywhere until when i saw a testimony of how Dr_Ogodo1 herbal mixture cream have been helping people regarding their sex life, so i decided to give him a try and to my greatest surprise in less than one week of taking the herbs my penis grow to 8 inches i couldn't believe my eyes and as i speak now my penis is now 8 inches and i do not have week erection again. I can make love to my wife longer in bed. And my marriage is now stable,my wife now enjoy me very well in bed.
    * He can also bring back your Ex
    *Love spell
    * Herpes virus diseases
    *Diabetes
    *Aid virus
    *STD/HSV
    *Cancer/typhoid E.t.c you contact him through the mail. Ogodoherbalhomesolution@gmail.com
    You can also WhatsApp him on +2349044680467

    ReplyDelete
  30. Good news this is to everyone out there with different health challenges, as I know there are still a lot of people suffering from different health issues and are therefore looking for solutions. I bring you Good news. There is a man called Dr ehiaguna a herbal practitioner who helped cure me from HSV (2), i had suffered from this diseases for the past 5 years and i have spent so much money trying to survive from it. I got my healing by taking the herbal medicine Dr ehiaguna sent to me to drink for about 14 days . 3 days after completion of the dosage, I went for a medical checkup and I was tested free from HSV. all thanks to God for leading me to Dr ehiaguna who was able to cure me completely from this deadly diseases, I’m sharing this so that other people can know of this great healer called Dr ehiaguna because I got to know him through elizabeth who he cured from HIV. I was made to understand that he can cure several other deadly diseases and infections. Don’t die in ignorance or silent and don’t let that illness take your life. Contact Dr ehiaguna through his email  drehiaguna@gmail.com You can also whatsapp/call him on:+2348073908953 . Visit his website  https://effectiveherbalcurehome.blogspot.com/   He cure all forms of disease {1}HIV/AIDS {2}DIABETES {3}EPILEPSY {4} BLOOD CANCER {5} HPV {6} BRAIN TUMOR {7} HEPATITIS {8}COPD{9} SICKLE AND ANAEMIA.etc Be kind enough to share as you received.  

    ReplyDelete
  31. I want every herpes patient to please read my testimony, my name is SARAH MORGAN and I am from California in the USA, I contracted genital herpes from my ex boyfriend who never had any symptoms. I have had it for 4 months now., and it has affected my life. I have told my boyfriends who I trusted about it and I have never had a bad reaction, it has affected my new relationships with Smith and people think herpes is really a minor skin irritation herpes has a long term effects on health. The stigma attached to this virus by ignorant people is ridiculous. Most people have herpes in one form or another. I would like to advise people on how I get rid of my herpes and I was reading a comment on the internet,and I saw a testimony posted by a woman from Germany that she get rid of her herpes with the help of DR AHKIGBE and so I was so happy when I saw that post, that his herbal medication is free and I quickly collected the herbal doctor email and I email him within 3 hr he respond to my email and I explain things to him he told me not to worry that he is going to cure me totally with his herbal medicine he only request for little money that he will use to buy the items for the preparation of the herbal medicine, wish I send to him because the pain was too much for me to bear and after some days he told me that he has prepare the herbal medicine, that I should send him my address that he want to send it to me via DHL or FED-EX that was how I got the herbal medication and I use it as I was told and after few days I found out that my herpes was no more, i went to hospital for confirmation and it was true really that was how i got my cured. DR AKHIGBE also cure other deadly diseases like,  HIV/AIDS, HERPES, DIABETES,CANCER, ALS, ASTHMA, WEAK BODY,  HEPATITIS A&B, DENGUE FEVER, RABIES, MARBURG  DISEASE, THYROID,  HYPEROPIA, SMALLPOX, ARTHRITIS, MENINGITIS ,LUPUS, EPILEPSY, CHRONIC DISEASE, DICK AND BREAST ENLARGEMENT, MALARIA, BACTERIAL DIARRHEA HEART DISEASE., JOINT PAIN, STOMACH PAIN,  DENGUE SCHIZOPHRENIA, POLIO,MULTIPLES SCLEROSIS, HIGH BLOOD PRESSURE,  SORENESS/INJURY,  VAGINAL  DISCHARGE,  CHANCROID, DEPRESSION, TUBERCULOSIS, ALZHEIMER , PENIS ENLARGEMENT, VAIN, PARKINSON'S, to get your rid kindly via his email:   drrealakhigbe@gmail.com    contact his number:  +2349010754824           website:  https://drrealakhigbe.weebly.com       you can still write me on Instagram to get more information. on Sarah Morgan 06.

    ReplyDelete

  32. I am so Happy to be writing this article in here, i am here to explore blogs forum about the wonderful and most safe cure for HERPES SIMPLEX VIRUS.I was positive to the Virus called HERPES and i lost hope completely because i was rejected even by my closet friends. i searched online to know and inquire about cure for HERPES and i saw testimony about DR Ebhota online on how he cured so many persons from Herpes Disease so i decided to contact the great herbalist because i know that nature has the power to heal everything. i contacted him to know how he can help me and he told me never to worry that he will help me with the natural herbs from God! after 2 days of contacting him, he told me that the cure has been ready and he sent it to me via FEDEX or DHL and it got to me after 4 days! i used the medicine as he instructed me (MORNING and EVENING) and i was cured! its really like a dream but i'm so happy! that's the reason i decided to also add more comment of Him so that more people can be saved just like me! and if you need his help,contact his Email: (drebhotasoltion@gmail.com) You can contact him on WhatsApp +2348089535482 He also have the herb to cure difference cure for any sickness (1) HERPES,
    (2) DIABETES,
    (3) HIV&AIDS,
    (4) URINARY TRACT INFECTION,
    (5) HEPATITIS B,
    (6) IMPOTENCE,
    (7) BARENESS/INFERTILITY
    (8) DIARRHEA
    (9) ASTHMA..

    ReplyDelete
  33. I Want To Appreciate Dr OYAGU for his great deeds, I Was Diagnosed With type 2 Herpes Virus Last year, And I Was Looking For Solution To Be Cured Luckily I Saw Testimonies On How Dr OYAGU Cure Herpes Virus I Decided To Contact Dr OYAGU I Contacted Him He Prepared A Herbal Medicine Portion And Sent It To Me, I Started The Herbal Medicine For My Health. He Gave Me Step By Step Instructions On How To Apply It, When I Applied It As Instructed, I Was Cured Of This Deadly Herpes Within 2 weeks, I Am Now Herpes Negative. My Brother And Sister I No That There Are So Many People That Have The Same Herpes Virus Please contact Dr OYAGU To Help You Too, And Help Me To Thank Dr OYAGU For Cure Me, I’m Cured By Dr. OYAGU Herbal Medicine, His Contact Email:oyaguherbalhome@gmail.com or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhomeOr Cell Whatsapp Number +2348101755322 thank you

    ReplyDelete
  34. First, I want to complement this site administrator for creating this platform I frequently get cold sores(which lasts for 5-6 days on my lips and its painful both physically and mentally) .. As a bodybuilder, I basically get outbreak due to the daily pressure and heat in the body.. Recently I stumbled upon my salvation. I found a herbal remedy to cure hsv completely, in just 4weeks ..

    I got to know about Dr Utu after seeing a lady's testimony online. I reached the doctor and I ordered for herpes herbal medicine. He sent the herbs to me and I started making use of them. In just 2days, the cold sores are cleared. 4weeks after medication, I went to the hospital for test of cold sores and it was completely NEGATIVE.

    By following every prescription, i was able to cure my cold sore in less than 4weeks. 100% natural herbs no after effect.
    Note: Please do let others know if this work for you.
    The doctor's email is drutuherbalcure@gmail.com
    Note : follow all instructions accordingly..

    If you are sure that you have benefited from this African herbs be courageous to inform others.
    Thanks in advance

    ReplyDelete
  35. I want to use this opportunity to thank Dr Oyagu for the grate work he has done for me and my cousin sister, I was cured from herpes virus through his herbal remedy and my cousin sisters was also cured from Cancer. This is a grate testimony for what Dr Oyagu Has done for me and my entire generation. I’m giving this testimony so that those of you out there can contact him while he keeps saving my relatives. Send him a Dm and get a better health totally. Contact him via WhatsApp +2348101755322 or Email address:oyaguherbalhome@gmail.com you can also reach him on his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome

    ReplyDelete
  36. Hello Am From The UK , I want to write a little testimony about the good work of doctor Oyagu who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc Oyagu email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address oyaguherbalhome@gmail.com or text him on what's app, phone number +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Dr Oyagu also has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE AND MANY MORE. I oblige everyone to contact this powerful herbalist Dr Oyagu and be free from suffering.

    ReplyDelete
  37. HOW DR AZIBA HELPED ME ENLARGE MY PENIS SIZE FROM 3.5 INCHES TO 11INCHES LONG AND 8.0 GROWTH WhatsAPP DR [ +2348100368288 ]
    I got married 2 years ago and it just seemed that there was no excitement in my sex life. My dysfunction to perform to the best of my abilities in bed made it harder for my wife and me to have a good time during sex. And i was having the feelings that she may decide to get a divorce one day. I knew something had to be done in order to improve my sex life and to save my marriage because my marriage was already falling apart, so when i was on my Facebook page i came across a story of how Dr Aziba helped him enlarged his penis to 8ins better.so i Immediately copied the Email address of the Dr and explained to him my problem, he gave me some simply instruction which i must follow and i did easily and my friends Today, i am the happiest man on Earth, All Thanks to Dr Aziba for saving my marriage and making me a real man today. I asked him about some popular diseases like HERPES,HIV AIDS,HEPATITES,DIABETIS, CANCERS, WART, HPV etc.. which are affecting most people he said there is no disease without a cure, he has it all...You can as well reach the Dr below for help on your problem, for he has the solution to all...
    Email:[ PRIESTAZIBASOLUTIONCENTER@GMAIL COM ]
    WhatsAPP DR [ +2348100368288 ]


















    HOW DR AZIBA HELPED ME ENLARGE MY PENIS SIZE FROM 3.5 INCHES TO 11INCHES LONG AND 8.0 GROWTH WhatsAPP DR [ +2348100368288 ]
    I got married 2 years ago and it just seemed that there was no excitement in my sex life. My dysfunction to perform to the best of my abilities in bed made it harder for my wife and me to have a good time during sex. And i was having the feelings that she may decide to get a divorce one day. I knew something had to be done in order to improve my sex life and to save my marriage because my marriage was already falling apart, so when i was on my Facebook page i came across a story of how Dr Aziba helped him enlarged his penis to 8ins better.so i Immediately copied the Email address of the Dr and explained to him my problem, he gave me some simply instruction which i must follow and i did easily and my friends Today, i am the happiest man on Earth, All Thanks to Dr Aziba for saving my marriage and making me a real man today. I asked him about some popular diseases like HERPES,HIV AIDS,HEPATITES,DIABETIS, CANCERS, WART, HPV etc.. which are affecting most people he said there is no disease without a cure, he has it all...You can as well reach the Dr below for help on your problem, for he has the solution to all...
    Email:[ PRIESTAZIBASOLUTIONCENTER@GMAIL COM ]
    WhatsAPP DR [ +2348100368288 ]

    ReplyDelete
  38. I want to tell everyone how i was cured of HERPES Simplex Virus by a Doctor called Dr Oje Abacha, i visited different hospital but there was no solution until one day i was browsing through the Internet searching for solution and i saw comment of people talking about how Dr Oje Abacha cured them. When I contacted him he gave me hope and sent a Herbal medicine to me that I took and it seriously worked for me,i'm free now without any problem, my HERPES result came out negative. I pray for you Dr Oje Abacha God will give you everlasting life, you shall not die before your time for being a sincere and great man. I'm so happy. You can also  email him at:  oje.herbalsolutioncentre@gmail.com  Or contact him on whatsapp  via +2348159734766 . or Visit his website  https://ojeherbalsolutioncentre.wordpress.com.

    ReplyDelete
  39. I'm here to testify about what DR. OYAGU did for me. I have been suffering from (GENITAL HERPES VIRUS) disease for the past 2 years and had constant pain and inching, especially in my private part. During the first year, I had faith in God that i would be cured someday.This disease started circulating all over my body and I have been taking treatment from my doctor, few weeks ago I came across a testimony of Shayla Bosschart on the internet testifying about a Man called DR. OYAGU on how he cured her from 7 years HSV 2. And she also gave the email address of this man, advise anybody to contact him for help on any kind of diseases that he would be of help, so I emailed him telling him about my (HSV 2) he told me not to worry that I was going to be cured!! Well, I never doubted him I have faith he can cure me too,, DR. OYAGU prepared and sent me Healing Soap, roots and herbs which I took. In the first one week, I started experiencing changes all over me, after two weeks of using his Roots/ Herbs, Oil and Soap, I was totally cured. no more inching , pain on me anymore as DR. OYAGU assured me. After some time I went to my doctor to do another test behold the result came out negative. So friends my advise is if you have such disease or know anyone who suffers from it or any other disease like HPV, HBV, HIV, ALS, HBP, CANCER etc. you can contact DR. OYAGU for help via email} oyaguherbalhome@gmail.com whatsaap him through his mobile} +2348101755322. , Thanks once again DR. OYAGU for making me a happy woman again.

    ReplyDelete
  40. My name is Denial Edward, I want to thank Dr. Patrick who cast a SPELL that helped my family win a court case that would have sentenced my brother for life, who was charged with murder. My family and I are totally grateful. You can also contact him if you have a similar challenge on the email: drpatrickspelltample2001@gmail.com, he also cast a spell to win the lottery, get your ex-lover back, promotion spell, luck spell, coronavirus COVID 19, hiv cure / AIDS, cancer, spell to have children, etc.

    ReplyDelete
  41. Website: (https://emutemple.wordpress.com/  I was born and raised in a small town on Long Island in New York. I live there today with my wife and three wonderful children. I want to testify of how I was cured from prostate cancer by Dr Emu. I was on hormone therapy for advanced prostate cancer. At first I was having lots of signs and symptoms like excessive urine at midnight and also I was experiencing dribbling of urine all the time I was going through hell I search for a cure☑ on the internet I came to know about Dr Emu I saw lots of testimonies of how he cures diseases like *Prostate Cancer *Herpes  Virus *Genital herpes *Hiv/Aids*Breast Cancer and more am happy am cured thanks to Dr Emu I recommend Dr Emu roots and herbs as best natural herbal medicine with no side effects. For consultation and more contact him via Email: (emutemple@gmail.com) (emutemple@yahoo.com) WhatsApp or call +2347012841542 

    ReplyDelete
  42. I am here to give my testimony about Dr Ebhota who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr Ehbota cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so happy as i am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ebhota healing herbal cure of all kinds of sickness you may have like
    (1) CANCER,
    (2) DIABETES,
    (3) HIV&AIDS,
    (4) URINARY TRACT INFECTION,
    (5) CANCER,
    (6) IMPOTENCE,
    (7) BARENESS/INFERTILITY
    (8) DIARRHEA
    (9) ASTHMA
    (10)SIMPLEX HERPES AND GENITAL
    (11)COLD SORE
    HERPES. he also cure my friend from cervical cancer Email: drebhotasoultion@gmail.com or whatsapp him on +2348089535482

    ReplyDelete
  43. I am here to give my testimony about Dr Ebhota who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr Ehbota cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so happy as i am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ebhota healing herbal cure of all kinds of sickness you may have like (1) CANCER,(2) DIABETES,(3) HIV&AIDS,(4) URINARY TRACT INFECTION,(5) CANCER,(6) IMPOTENCE,(7) BARENESS/INFERTILITY(8) DIARRHEA(9) ASTHMA(10)SIMPLEX HERPES AND GENITAL(11)COLD SOREHERPES. he also cure my friend from cervical cancer Email: drebhotasoultion@gmail.com or whatsapp him on +2348089535482

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  44. My Ex-boyfriend left me and my daughter since 6 weeks ago after, I tried almost  everything  to restore my relationship and I was disappointed. None was working out, A week later I saw Dr. Paul website and after I contact Dr.  Paul   for help, everything automatically change, my sadness became joy, smile was all over my face, everything happened very fast  and the result was effective. Dr  Paul   is the only  spell caster who i had success with, my boyfriend called me and said she wanted us to come back and also want to marry me. he came back to me begging me to accept him back and this happened Due to the help of Dr.  Paul  . I Am so happy that I have my boyfriend back. Contact Dr.  Paul   now for an urgent and fast love spell to win your relationship back. Email him: astoriashrine@gmail.com

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  45. Hello everyone i want to share little experience about a herbal Doctor WHO GOT ME CURE of #HPV I was infected by my partner, we dated for so many years but I never knew whenever he went to work,on his way coming back all he do is womanizing but I never expected him doing such thing because he was calm and humble unknown to he was pretending that’s how he was infected with HPV and transmitted it to me.
    Furthermore DEPRESSIONS and FRUSTRATION took over me when I was confirm #HPV all this years have been so angry because I didn’t deserve it from my partner well all thanks to a friend who recommended a herbal Doctor to me and got me cure with his herbal medicine.he also specialize in treating all kinds of virus diseases.
    You can reach him Email or WhatsApp
    Email đŸ“§{ogodoherbalhomesolution@gmail.com}
    WHATSAPP {+2348124774363}
    https://www.facebook.com/oogoherbalhomesolution/

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  46. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442This testimonial serves as an expression of my gratitude.
    He also have a herbal cure for COLD SORE,
    SHINGLES,
    CANCER(Cannabis Oil),
    HPV,
    ASTHMA,
    IMPOTENCE,
    BARENESS/INFERTILITY ..
    Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

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