Tuesday, May 23, 2017

GRCz11 – the latest zebrafish reference genome assembly



After 2.5 years of assembly curation, the GRC is proud to present the new zebrafish reference genome assembly, GRCz11.

This latest assembly has been refined by the addition of nearly 1000 finished clone sequences and by the resolution of more than 400 assembly issues. This resulted in a significant reduction in scaffold numbers (3399 to 1905) and increase in scaffold N50 (2.18 Mb to 7.5 Mb) whilst the overall genome size was not affected.  Figure 1 shows an overview of contig and scaffold N50s over time, indicating the advance in assembly curation.



Figure 1: Contig vs. scaffold N50s for zebrafish reference genome assemblies. Release dates: Zv7: 2008, Zv8: 2009, Zv9: 2010, GRCz10: 2014, GRCz11: 2017.


Alignments of 16133 RefSeq sequences showed a further improvement over past assemblies: only 31 sequences remained not found (down from 34), 105 transcripts are still split between locations (down from 205) and only 441 exhibit less than 95% CDS coverage (down from 566). Figure 2 shows an example of an improved region, correcting the representation of two genes.



Figure 2: gEVAL screenshot of the supt4h1 gene (red arrow) in GRCz10 (top) and GRCz11 (bottom). In GRCz10 the supt4h1 gene on chromosome 5 is incomplete, missing its first exon, and surrounded by a truncated supplicated copy of rnf150b (blue arrow). In GRCz11, the supt4h1 gene is complete and neighbouring the hsf5 gene, as seen in other vertebrates, whereas the rnf150b gene is now complete and located singularly on chromosome 23. gEVAL, the GRC’s genome assembly evaluation browser, indicates completeness of genes and other features via colours (green > 98% coverage, yellow = 50-98% coverage, red < 50% coverage).  


GRCz11 was built as described previously using clone sequences ordered and oriented according to genetic markers and BioNano data, the latter greatly influencing the scaffolding. Remaining gaps were filled with selected contigs from whole genome sequencing assemblies, mainly WGS31, and in a few cases, WGS32.

For the first time in a zebrafish assembly, GRCz11 also features alternate loci scaffolds (ALT_REF_LOCI). The alternate loci represent variant sequence representations for certain genomic regions. They were selected from a pool of 1895 finished clones that were found to inhabit an assembly region already occupied by clone sequence and were therefore not included in the primary chromosomal path. All surplus clones that exhibited at least 5 kb of unique sequence not present in the primary chromosomal path were added to the assembly as alternate loci scaffolds, totaling 186 Mb of additional sequence in 1150 clones. The alignments of the alternate loci scaffolds to the primary chromosomal path are also included in the GRCz11 assembly to provide the chromosome context for these alternate sequences. The alternate loci will be represented in genome browsers in the same way as human and mouse ALT_REF_LOCI. Additional smaller scale variation will be submitted to dbSNP/dbVAR/EVA.

After release of this assembly, within the GRC, the Sanger team is transferring the maintenance of the zebrafish reference to the new GRC member ZFIN. ZFIN will take on the future curation of the assembly, and invites user reports on assembly issues. Future updates to the assembly will be issued as patch releases, adding sequence but not impacting the chromosomal coordinates.

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    ReplyDelete
  4. First, I want to complement this page administrator for creating this platform for us to express our feelings. Herpes is a serious and recurring disease which can't be cured through drugs or injections by the American doctors but the best way to deal with herpes is by taking natural herbs medicine from DR. ISHIAKU the greatest herbalist doctor in the world and is only few American doctors that know about this herbal medicine from Dr ISHIAKU .. I have read about Dr ISHIAKU the great herbalist doctor from African who can cure disease with his powerful herbal medicine. for the people suffering from the following diseases, Herpes,HIV/Aids, Cancer, Also, Hepatitis, Diabetes, Hpv, Infections ETC should contact him for his herbal medicine because i am a living testimony and i was cured of herpes. Although, i sent him what he requested and he sent me his medicine through DHL courier delivery company which i took for some days and today when i went for test, i was tested herpes negative. you can reach him through his email: ishiakuherbalcure@gmail.com WhatsApp him at +2348180828544  

    ReplyDelete
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    ReplyDelete
  6. I visited different hospital but they gave me list of expensive drugs to treat the symptoms and never cured me. A closed friend of mine introduce me to a Herbal Doctor who cured her. I was scared to contact him because of what i experienced from fake doctors. she encouraged me and gave me his whatsapp contact,and i contacted him and i asked him series of questions, i also ask him to show me the results of those he have cured with his herbal drugs,and he gave me all the information i needed that gave me hope. He send a Herbal medicine to me that i took and it seriously worked for me. am now Hepatitis negative. God bless you for being a sincere and great man. Am so excited and am free from herpes virus. you can reach him via email;drshaibuabacha@gmail.com or whats-app/call on +2349051727475. He also cure several ailments like ; Mouth odour,  Diabetes, Stroke, Hypertension,  kidney problem, Ex back, Low sperm count, Weak Erection and HIV/Aids. Please do not hide you problems so your problems will not hide you.

    ReplyDelete


  7. I am so Happy to be writing this article in here, i am here to explore blogs forum about the wonderful and most safe cure for HERPES SIMPLEX VIRUS.I was positive to the Virus called HERPES and i lost hope completely because i was rejected even by my closet friends. i searched online to know and inquire about cure for HERPES and i saw testimony about DR Ebhota online on how he cured so many persons from Herpes Disease so i decided to contact the great herbalist because i know that nature has the power to heal everything. i contacted him to know how he can help me and he told me never to worry that he will help me with the natural herbs from God! after 2 days of contacting him, he told me that the cure has been ready and he sent it to me via FEDEX or DHL and it got to me after 4 days! i used the medicine as he instructed me (MORNING and EVENING) and i was cured! its really like a dream but i'm so happy! that's the reason i decided to also add more comment of Him so that more people can be saved just like me! and if you need his help,contact his Email: (drebhotasoltion@gmail.com) You can contact him on WhatsApp +2348089535482 He also have the herb to cure difference cure for any sickness (1) HERPES,
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    ReplyDelete
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  10. I want to share this wonderful testimony to the Good people all over the world on how I was able to Enlarge my Penis by Dr.ODOMA I was living a shameful life from my young age, just last month as I was browsing on the internet about Penis size and Enlargement Products, I saw a testimony of a Man called George , testifying of how he was able to get his penis Enlarged by Dr.odoma and I decided to also Email Dr.odoma for my small penis size and he quickly respond to me and gave me the normal instructions which i did and then he shipped the product to me here in the united state which i received in just 3 working days and today i am very happy because i started seeing positive changes in my penis size in just 7 days of use. Dr.ODOMA herbal product is the best recommended for you and to whomever suffering from this shame or having any other diseases as well should Contact this great herbal doctor via his Email : drodomasolutionhome20@gmail.com and WhatsApp Him on +2348100649947

    ReplyDelete
  11. I want to share this wonderful testimony to the Good people all over the world on how I was able to Enlarge my Penis by Dr.ODOMA I was living a shameful life from my young age, just last month as I was browsing on the internet about Penis size and Enlargement Products, I saw a testimony of a Man called George , testifying of how he was able to get his penis Enlarged by Dr.odoma and I decided to also Email Dr.odoma for my small penis size and he quickly respond to me and gave me the normal instructions which i did and then he shipped the product to me here in the united state which i received in just 3 working days and today i am very happy because i started seeing positive changes in my penis size in just 7 days of use. Dr.ODOMA herbal product is the best recommended for you and to whomever suffering from this shame or having any other diseases as well should Contact this great herbal doctor via his Email : drodomasolutionhome20@gmail.com and WhatsApp Him on +2348100649947

    ReplyDelete
  12. I am here to give my testimony about Dr Ebhota who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedies on HERPES and I saw comments of people talking about how Dr Ehbota cured them. When I contacted him he gave me hope and sent Herbal medicine to me that I took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so haas I am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ebhota healing herbal cure of all kinds of sickness you may have like
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    HERPES. he also cured my friend from cervical cancer Email: drebhotasoultion@gmail.com or whatsapp him on +2348089535482

    ReplyDelete
  13. HOW I GOT CURED FROM  HPV WARTS Greetings to the general public, i want to tell about how i was cured of HPV disease by a Doctor called DR MOMOH i was browsing through the Internet searching for remedy on HPV and i saw comment of people talking about how DR MOMOH cured them. I Was scared because i never believed in the Internet but i was convince to give him a try because i having no hope of been cured of HPV so i decided to contact him with his email that was listed on the comment when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HPV result came out negative. I pray for you God will give you everlasting life, you shall not die before your time for being a sincere and great man. Am so happy, you can also contact him if you have any problem Email: dr.momoh9@gmail.com , or whatsApp him on this number +2347083724098 1. LOVE SPELL 2. ALS CURE 3. HEPATITIS B 4. HIV/AIDS CURE 5. HERPES CURE 6 SPIRITUAL HEALERS

    ReplyDelete
  14. it"s obvious some patients with Herpes Virus are being enslaved to the antiviral and other supplementary Orthodox medicine just to help suppress the virus and not a cure. I have been with the virus since 2015 until I was introduced by a blogger who also narrated her story online on how she was cured of HPV after using Dr ALUDA Herbal Medicine. This is a year and 2 weeks since I was delivered from Herpes VIRUS. All thanks to God for using this Great herbalist to heal me. I have promised to keep telling good things about Dr ALUDA. Please feel free to share Your problems with him and don’t forget to tell him I did refer you to Him. Thanks. Write him on whatsapp +2348145636867 or mail him @ Draludaharbalhome @gmail. com He deals with Alzheimer virus, Cancer, HIV, Herpes, Genital, warts, ALS, HBV, UTI, Virginal infection, Genital, Wart, HPV, Hepatitis A/B, Good luck, HSV,  Cold Sores, Diabetes 1 & 2, Pregnancy, Ex back.

    ReplyDelete

  15. Am jeremiah, I am testifying about a great hebal man that cured my wife of hepatitis B and liver cirhosis. his name is Dr oniha. My wife was diagnose of hepatities two years ago, i almost spent all i had then, until i saw dr oniha recommendation online, and i call him, then he told me how to get the herb. You can call him on +2347089275769 or email him at dronihaspell@yahoo.com

    ReplyDelete
  16. All thanks to Dr Alli for curing my herpes virus/hpv with his herbal medicine, i do not have much to say but with all my life i will forever be grateful to him and God Almighty for using Dr Alli to reach me when i thought it was all over, today i am happy with my life again after the medical doctor have confirmed my HERPES SIMPLEX VIRUS / HPV of 5 is gone,i have never in my life believed that HERPES SIMPLEX VIRUS could be cured by herbal medicine. so i want to use this means to reach other persons who have this disease by testifying the power of Dr Alli that all hope is not lost yet, try and contact him by any means for any kind of disease with his email: ( Allispellhelp1@gmail.com ) or his whatsapp number +2348100772528 and get your healing

    ReplyDelete
  17. Are you having issues in your relationship, or finding it difficult to have a child of your own or are you the type having any diseases in your body. I will advice you to contact Dr. Abolo of Abolospell@gmail.com . The potency of his spell is so effective and can reunite broken relationship and can also make one get pregnant know matter the situation and not only that, he also has the power to cure all type of diseases within the period of 48hours.. Contact him today and thank me later.

    Contact details : Abolospell@gmail.com

    ReplyDelete
  18. My names are Julia moreton I’m a citizen of UK, My younger sister was sicking of
    breast cancer and her name is Florence   I and my family have been taking her
    to all kind of hospital over here(UK) still yet no good result. I decided to go to
    the internet and search for cancer cure so that was how I find a lady
    called Sophia Charlotte,   she testifies to the world about the goodness of a
    herbal man who has the roots and herbs to cure all kind of disease and the
    herbal man email was there. So I decided to contact the herbal man @ kokobiherbalremedycentre@gmail.com for my
    younger sister to see if he can cure her from the ailment that has befallen her(breast cancer). I contacted him and told him
    my problem he told me that I should not worry that my sister will be
    cure, he told me that there is a medicine that he is going to send give to me to cook and give it to my sister to drink for one week, so I ask how
    can I receive the cure that I am in UK, he told me
    That I will pay for the delivery service. The courier service can
    transport it to me so he told me the amount I will pay, so my dad paid for
    the delivery fee. two days later I receive the cure from the courier
    service so I used it as the herbal man instructed me to, before the week runs out, my sister cancer was healed and it was like a dream to me not
    knowing that it was physical I and my family were very happy about the
    miracle of Doctor kokobi. my dad wanted to pay him 5 million  dollars as token of our appreciation towards his good work but the
    herbal man declined the offer which I don't know why, he only say that I should tell the world about him
    and the miracle he perform so am here now to tell the world about him if
    you or your relative is having any kind of disease that you can't get a solution to from
    the hospital please contact kokobiherbalremedycentre@gmail.com or WhatsApp him
    +254746618873 or call him via his calling line +2348114448587 . And if you need more information about the doctor you can send me an mail @
    juliamoreton78@gmail.com

    ReplyDelete
  19. HERPES INFECTION CURE IS 100% AVAILABLE WITH THE HELP OF DR OGBERAESE HERBAL MEDICINE.Joy and happiness is all i can see around ever since i came in contact with this great man Called Dr Ogberaese. i complained bitterly to him about me having herpes only for him to tell me it’s a minor stuff. He told me he has cured thousands of people but i did not believe until he sent me the herbal medicine and i took it as instructed by this great man, only to go to the hospital after two weeks for another test and i was confirmed negative. For the first time in four years i was getting that result. i want to use this medium to thank this great man. His name is Dr Ogberaese , i came in with his email through a friend in USA called Edith Rose and ever since then my live has been full with laughter and great peace of mind. i urge you all with herpes or HSV1&2 to contact him if you are willing to give him a chance. you can contact him through his email drogberaese54@gmail.com or you can also WhatsApp him +2348073818653

    ReplyDelete
  20. HERPES INFECTION CURE IS 100% AVAILABLE WITH THE HELP OF DR OGBERAESE HERBAL MEDICINE.Joy and happiness is all i can see around ever since i came in contact with this great man Called Dr Ogberaese. i complained bitterly to him about me having herpes only for him to tell me it’s a minor stuff. He told me he has cured thousands of people but i did not believe until he sent me the herbal medicine and i took it as instructed by this great man, only to go to the hospital after two weeks for another test and i was confirmed negative. For the first time in four years i was getting that result. i want to use this medium to thank this great man. His name is Dr Ogberaese , i came in with his email through a friend in USA called Edith Rose and ever since then my live has been full with laughter and great peace of mind. i urge you all with herpes or HSV1&2 to contact him if you are willing to give him a chance. you can contact him through his email drogberaese54@gmail.com or you can also WhatsApp him +2348073818653

    ReplyDelete
  21. My name is Thomas White, from United states, i have been Herpes positive and it is just like been through hell; well special thanks to God almighty for using Dr Twaha, the greatest Herbal doctor in curing my herpes disease. I was diagnosed of this disease in the year 2004. Although I was taking my medications I was not myself. Until a few weeks ago. My friend came to me and told me that he saw many testimonies on how Dr. Twaha  cured people Herpes disease. Although I never believe in herbs, I said to myself "seeing is believing" I took his name and searched it on GOOGLE, I saw many testimonies myself. Quickly, I copied his email: drlregbeyen10000@gmail.com I emailed him, he gave me a form to fill which I did, then he sent me a message  and told me that he required some items in which he will use in preparing the cure for me and he told me that it will cost me some money which i sent to him because I need the cure urgent,  He bought the items and prepared a cure for me, then he sent the parcel to me through courier delivery service. I paid the courier services, I got my parcel, it was a bottle of ointment as described by Dr,Twaha After taking the ointment he told me to do, the herpes test and the result came out negative. Quickly I messaged him and told him what happened he congratulated me. I promised to tell the world about him. You can contact him with any problem  via email: ( drlregbeyen10000@gmail.com ) or contact him on WhatsApp +2349038518881 thank you Dr Twaha

    ReplyDelete
  22. Good news this is to everyone out there with different health challenges, as I know there are still a lot of people suffering from different health issues and are therefore looking for solutions. I bring you Good news. There is a man called Dr ehiaguna a herbal practitioner who helped cure me from HSV (2), i had suffered from this diseases for the past 5 years and i have spent so much money trying to survive from it. I got my healing by taking the herbal medicine Dr ehiaguna sent to me to drink for about 14 days . 3 days after completion of the dosage, I went for a medical checkup and I was tested free from HSV. all thanks to God for leading me to Dr ehiaguna who was able to cure me completely from this deadly diseases, I’m sharing this so that other people can know of this great healer called Dr ehiaguna because I got to know him through elizabeth who he cured from HIV. I was made to understand that he can cure several other deadly diseases and infections. Don’t die in ignorance or silent and don’t let that illness take your life. Contact Dr ehiaguna through his email drehiaguna@gmail.com You can also whatsapp/call him on:+2348073908953 .He cure all forms of disease {1}HIV/AIDS {2}DIABETES {3}EPILEPSY {4} BLOOD CANCER {5} HPV {6} BRAIN TUMOR {7} HEPATITIS {8}COPD{9} SICKLE AND ANAEMIA.etc Be kind enough to share as you received.

    ReplyDelete
  23. Good news this is to everyone out there with different health challenges, as I know there are still a lot of people suffering from different health issues and are therefore looking for solutions. I bring you Good news. There is a man called Dr ehiaguna a herbal practitioner who helped cure me from HSV (2), i had suffered from this diseases for the past 5 years and i have spent so much money trying to survive from it. I got my healing by taking the herbal medicine Dr ehiaguna sent to me to drink for about 14 days . 3 days after completion of the dosage, I went for a medical checkup and I was tested free from HSV. all thanks to God for leading me to Dr ehiaguna who was able to cure me completely from this deadly diseases, I’m sharing this so that other people can know of this great healer called Dr ehiaguna because I got to know him through elizabeth who he cured from HIV. I was made to understand that he can cure several other deadly diseases and infections. Don’t die in ignorance or silent and don’t let that illness take your life. Contact Dr ehiaguna through his email drehiaguna@gmail.com You can also whatsapp/call him on:+2348073908953 .He cure all forms of disease {1}HIV/AIDS {2}DIABETES {3}EPILEPSY {4} BLOOD CANCER {5} HPV {6} BRAIN TUMOR {7} HEPATITIS {8}COPD{9} SICKLE AND ANAEMIA.etc Be kind enough to share as you received.

    ReplyDelete
  24. HOW I GOT CURED FROM HERPES VIRUS I was diagnosed of HERPES virus and i have tried all I can to get cured but all to no avail,,until I saw a post in a health forum about a herbalist man who prepare herbal medications to cure all kind of diseases including HERPES virus,at first I doubted if it was real but decided to give it a try,when I contacted this herbalist via his email and he prepared a HERPES herbal cure and sent it to me via UPS delivery company service, when I received this herbal medicine, he gave me step by direction on how to apply it,after applying theUPS delivery company service, when I received this herbal medicine, he gave me step by direction on how to apply it,after applying the. way I was instructed,I was totally cured of this deadly disease called HERPES, all thanks to Dr oboh.Email obohesan@gmail.com this great herbal doctor via WhatsApp+2348089281017 HE CAN ALSO CURE SICKNESS LIKE{1}HIV/AIDS
    {2}DIABETES
    {3}EPILEPSY
    {4} BLOOD CANCER
    {5} HPV
    {6} ALS
    {7} HEPATITIS
    {8}LOVE SPELL
    {9} SICKLE AND ANAEMIA..
    or via whatsapp +2348089281017

    ReplyDelete
  25. FINALLY FREE FROM THE HERPES VIRUS. what a miracle I never believe there is a cure because my doctor tested me positive for herpes and she told me that there is no cure, I am very happy today that I have a free life without this herpes virus, I can remember a few months ago when I cried at night and day that I can't cure this herpes, I found this herbal man called Dr. Odion on the internet, when I was doing research to treat shingles, I contacted him to find out if I could get it help from this herpes virus, i was so surprised when he told me that he cures herbs and he sent me the herb in less than 5 days, i was so happy when i get someone who gives me hope that he can cure me . I took the herbs for only 2 weeks, when I went for a test, after I took the herbs, I realized that I was cured, I was so happy and surprised, I want to use this opportunity to inform you that there is a cured herpes virus that you can also contact him for help as soon as possible so you can get rid of this herpes once and for all all you can contact him with this email: drodionherbalhome12@gmail.com or whatsApp with him on +2349019421176 You can also so contact him for any disease and virus as he has herbal remedies. 1 HIV / AIDS /HERPES / CANCER / HEPATITIS 6 DIABETES / HIGH BLOOD PRESSURE, ..... THANK YOU DR.

    ReplyDelete
  26. To be honest with doctor Twaha herbal medicine you are free from herpes virus disease we bless god almighty for sending Dr. Twaha Herbal Home to the world to help people cure this disease am so happy to found a good man like this God keep blessing you for me if you also need help from this good man kindly WhatsApp him via +2349038518881 or email him drlregbeyen10000@gmail.com

    ReplyDelete
  27. I was diagnosed as HEPATITIS B carrier in 2013 with fibrosis of the
    liver already present. I started on antiviral medications which
    reduced the viral load initially. After a couple of years the virus
    became resistant. I started on HEPATITIS B Herbal treatment from
    ULTIMATE LIFE CLINIC (www.ultimatelifeclinic.com) in March, 2020. Their
    treatment totally reversed the virus. I did another blood test after
    the 6 months long treatment and tested negative to the virus. Amazing
    treatment! This treatment is a breakthrough for all HBV carriers.

    ReplyDelete
  28. Dr. Ehimare has really made me so much believe in him by getting me cured of Herpes with his herbal medicine. I have been a Herpes virus patient for some time now, I have been running here and there looking for help/cure but none could help. I ran into a testimony by someone who testified how she was cured of a terrible disease by Dr. Ehimare, so I decided to contact the herbal doctor. I explained to him, and he said he can cure me. We started the process and he prepared herbs and sent them to me in my country and gave me directions on how I am to use them. I followed his directions and now To God be the glory he could cure me with his herbal mixture. I really appreciate you Dr. Ehimare for bringing back happiness to my life again. thanks you so much friends that has join me to thank Dr. Ehimare for what he has actually done for me i pray to you all for a good life and property, his contact. Email address drehimare3@gmail.com , via whats-app +1 (267) 691-1087 Thanks Dr. Ehimare for your help in my life and family... and most especially to you Dr. THANKS. 

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  29. Hello everyone , I want to write a little testimony about the good work of doctor Abolo who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this dr Abolo email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address draboloherbalhome@gmail.com or text him on what's app, phone number +2347044725611 and he has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE.HEPATITS.E.T.C I BEG everyone to contact this powerful herbal list Dr Abolo and be free from suffering

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  30. Hello, everyone! I,m here to explore blogs and forum about wonderful and most safe cure for (Herpes Virus).I was positive to the deadly virus called herpes and i lost hope because i was out casted and rejected even by my closet friends.i searched on-line to know and enquirer about cure for Herpes and i read someone testimony on how he was cured from Herpes so i decided to contact the same herbalist because i know that nature has the power to heal everything.i contacted him to know how he can help me and he told me never to worry that he will heal me with the natural herbs from God!after 2 days of contacting him, he told me that the cure has been ready and he sent it to me via DHL and it got to me after 3 days!i used the med as he instructed me (MORNING and EVENING) and i was cured!its really like a dream but i am so happy!thats the reason i decided to also add more comment of Him so that more can be saved just like me!and if you need his help, you can email him on drehimare3@gmail.com, or whatsapp +1 (267) 691-1087 I,m neme amber and you can get in touch with me via drehimare3@gmail.com..Contact him for help at Herpes virus HIV/AIDS CANCER COPD BRAIN TUMOR All kind of virus and disease Dr Ehimare

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  32. Hi everyone, 2years ago my doctor told me I was diagnosed with HSV2. I was heart broken, How- ever that same years i found a doctor called Dr ubarlo who helped me to cured the virus with natural herbal treatment per- manently. thanks to Dr ubarlo for his natural herbal treatment that eliminated this virus out in my body forever. his contact of him through Instagram page. for any incur- able virus THESE ARE THE DISEASES CURED BY dr ubarlo 1) HERPES 2) HIV / AIDS 3) lupus 4) CANCER 5) EPILEPSY 6) DIABETES 7) ERECTILE 8) CANCER CURE 9) asthma 10) RIGGING EARS 11) Gout 12) pregnancy 13) Hepatitis ABC 14) Weight loss 15) fibroids you can also contact him on his email drubarlohome@gmail.com or Whatsapp +2348119508814

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  35. HOW I GOT HEAL FROM HERPES SIMPLEX VIRUS BY DR ISHIAKU HERBS  
    The greatest Joy in my life today is that i have been cured of Herpes by a Doctor called Dr. Ishiaku, I was infected with HERPES Disease in 2019, i went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution,so that my body can be okay. Until one faithful day as I was browsing on the internet I saw a testimony on how Dr. ishiaku has helped people in curing Herpes and other diseases, quickly copied his WhatsApp number so I contacted him for a solution for my Herpes disease, So Dr. Ishiaku told me that his going to prepare his herbal medicine for my health which he sent to me, luckily after 2week my Herpes was cured. Dr Ishiaku is well recognized as one of the best herbalist doctor in Africa, you don't have to be sad anymore or cry anymore on this disease when the cure is available. The medicine has NO SIDE EFFECT,there's no special diet when taking the medicine. He also cure  ALS, CANCER, HPV, DIABETES and lots more.
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